Recombinant Full Length Human TKT Protein, C-Flag-tagged

• Cat.No.: TKT-2124HFL
• Product Overview: Recombinant Full Length Human TKT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 67.7 kDa
• AA Sequence: MESYHKPDQQKLQALKDTANRLRIS SIQATTAAGSGHPTSCCSAAEIMAV LFFHTMRYKSQDPRNPHNDR FVLSKGHAAPILYAVWAEAGFLAEA ELLNLRKISSDLDGHPVPKQAFTDV ATGSLGQGLGAACGMAYTGK YFDKASYRVYCLLGDGELSEGSVWE AMAFASIYKLDNLVAILDINRLGQS DPAPLQHQMDIYQKRCEAFG WHAIIVDGHSVEELCKAFGQAKHQP TAIIAKTFKGRGITGVEDKESWHGK PLPKNMAEQIIQEIYSQIQS KKKILATPPQEDAPSVDIANIRMPS LPSYKVGDKIATRKAYGQALAKLGH ASDRIIALDGDTKNSTFSEI FKKEHPDRFIECYIAEQNMVSIAVG CATRNRTVPFCSTFAAFFTRAFDQI RMAAISESNINLCGSHCGVS IGEDGPSQMALEDLAMFRSVPTSTV FYPSDGVATEKAVELAANTKGICFI RTSRPENAIIYNNNEDFQVG QAKVVLKSKDDQVTVIGAGVTLHEA LAAAELLKKEKINIRVLDPFTIKPL DRKLILDSARATKGRILTVE DHYYEGGIGEAVSSAVVGEPGITVT HLAVNRVPRSGKPAELLKMFGIDRD AIAQAVRGLITKA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway

Gene Information

• Gene Name: TKT transketolase [ Homo sapiens (human) ]
• Official Symbol: TKT
• Synonyms: TK; TKT1; SDDHD; HEL107; HEL-S-48
• Gene ID: 7086
• mRNA Refseq: NM_001064.4
• Protein Refseq: NP_001055.1
• MIM: 606781
• UniProt ID: P29401