Recombinant Full Length Human TPI1 Protein, C-Flag-tagged

• Cat.No.: TPI1-1400HFL
• Product Overview: Recombinant Full Length Human TPI1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 26.5 kDa
• AA Sequence: MAPSRKFFVGGNWKMNGRKQSLGEL IGTLNAAKVPADTEVVCAPPTAYID FARQKLDPKIAVAAQNCYKV TNGAFTGEISPGMIKDCGATWVVLG HSERRHVFGESDELIGQKVAHALAE GLGVIACIGEKLDEREAGIT EKVVFEQTKVIADNVKDWSKVVLAY EPVWAIGTGKTATPQQAQEVHEKLR GWLKSNVSDAVAQSTRIIYG
  GSVTGATCKELASQPDVDGFLVGGA SLKPEFVDIINAKQTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Inositol phosphate metabolism, Metabolic pathways

Gene Information

• Gene Name: TPI1 triosephosphate isomerase 1 [ Homo sapiens (human) ]
• Official Symbol: TPI1
• Synonyms: TIM; TPI; TPID; HEL-S-49
• Gene ID: 7167
• mRNA Refseq: NM_000365.6
• Protein Refseq: NP_000356.1
• MIM: 190450
• UniProt ID: P60174