| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
1-494 aa |
| Description : |
The protein encoded by this gene converts UDP-glucose to UDP-glucuronate, a key component in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosaminoglycans are common constituents of the extracellular matrix and play roles in signal transduction, cell migration, and cancer growth and metastasis. The encoded protein is also involved in the detoxification of endogenous and exogenous compounds through the UDP-glucuronosyltransferase pathway. Mutations in this gene are associated with developmental and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. |
| Form : |
Liquid |
| Bio-activity : |
Specific Activity: > 2500 pmol/min/ug |
| Molecular Mass : |
59.5 kDa (533aa including His-tag), confirmed by MALDI-TOF |
| AASequence : |
FEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQHSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQVETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV |
| Purity : |
> 95% (by SDS-PAGE) |
| Applications : |
SDS-PAGE, Enzyme Activity |
| Storage : |
+2 to +8 centigrade for 1 week; -20 to -80 centigrade long term. Avoid repeated freeze-thaw cycles. |
| Concentration : |
0.5 mg/ml (Bradford assay) |
| EC : |
1.1.1.22 |
| CAS : |
9028-26-6 |
| Unit definition : |
One unit is defined as the amount of enzyme that converts 1.0 pmol of UDP-glucose to UDP-glucuronate per minute at 37 centigrade, pH 8.7. |
| Storage Buffer : |
20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 1mM EDTA |
