Recombinant Full Length Human UNC45B Protein, C-Flag-tagged

• Cat.No.: UNC45B-1933HFL
• Product Overview: Recombinant Full Length Human UNC45B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 103.6 kDa
• AA Sequence: MAEVEAVQLKEEGNRHFQLQDYKAA TNSYSQALKLTKDKALLATLYRNRA ACGLKTESYIQAASDASRAI DINSSDIKALYRRCQALEHLGKLDQ AFKDVQRCATLEPRNQNFQEMLRRL NTSIQEKLRVQFSTDSRVQK MFEILLDENSEADKREKAANNLIVL GREEAGAEKIFQNNGVALLLQLLDT KKPELVLAAVRTLSGMCSGH QARATVILHAVRIDRICSLMAVENE EMSLAVCNLLQAIIDSLSGEDKREH RGKEEALVLDTKKDLKQITS HLLDMLVSKKVSGQGRDQALNLLNK NVPRKDLAIHDNSRTIYVVDNGLRK ILKVVGQVPDLPSCLPLTDN TRMLASILINKLYDDLRCDPERDHF RKICEEYITGKFDPQDMDKNLNAIQ TVSGILQGPFDLGNQLLGLK GVMEMMVALCGSERETDQLVAVEAL IHASTKLSRATFIITNGVSLLKQIY KTTKNEKIKIRTLVGLCKLG SAGGTDYGLRQFAEGSTEKLAKQCR KWLCNMSIDTRTRRWAVEGLAYLTL DADVKDDFVQDVPALQAMFE LAKXXTSDKTILYSVATTLVNCTNS YDVKEVIPELVQLAKFSKQHVPEEH PKDKKDFIDMRVKRLLKAGV ISALACMVKADSAILTDQTKELLAR VFLALCDNPKDRGTIVAQGGGKALI PLALEGTDVGKVKAAHALAK IAAVSNPDIAFPGERVYEVVRPLVR LLDTQRDGLQNYEALLGLTNLSGRS DKLRQKIFKERALPDIENYM FENHDQLRQAATECMCNMVLHKEVQ ERFLADGNDRLKLVVLLCGEDDDKV QNAAAGALAMLTAAHKKLCL KMTQVTTQWLEILQRLCLHDQLSVQ HRGLVIAYNLLAADAELAKKLVESE LLEILTVVGKQEPDEKKAEV VQTARECLIKCMDYGFIKPVS TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: UNC45B unc-45 myosin chaperone B [ Homo sapiens (human) ]
• Official Symbol: UNC45B
• Synonyms: CMYA4; MFM11; UNC45; CTRCT43; SMUNC45; UNC-45B
• Gene ID: 146862
• mRNA Refseq: NM_173167.3
• Protein Refseq: NP_775259.1
• MIM: 611220
• UniProt ID: Q8IWX7