Recombinant Full Length Human UPF1 Protein, C-Flag-tagged

• Cat.No.: UPF1-193HFL
• Product Overview: Recombinant Full Length Human UPF1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 122.9 kDa
• AA Sequence: MSVEAYGPSSQTLTFLDTEEAELLG ADTQGSEFEFTDFTLPSQTQTPPGG PGGPGGGGAGGPGGAGAGAA AGQLDAQVGPEGILQNGAVDDSVAK TSQLLAELNFEEDEEDTYYTKDLPI HACSYCGIHDPACVVYCNTS KKWFCNGRGNTSGSHIVNHLVRAKC KEVTLHKDGPLGETVLECYNCGCRN VFLLGFIPAKADSVVVLLCR QPCASQSSLKDINWDSSQWQPLIQD RCFLSWLVKIPSEQEQLRARQITAQ QINKLEELWKENPSATLEDL EKPGVDEEPQHVLLRYEDAYQYQNI FGPLVKLEADYDKKLKESQTQDNIT VRWDLGLNKKRIAYFTLPKT DSDMRLMQGDEICLRYKGDLAPLWK GIGHVIKVPDNYGDEIAIELRSSVG APVEVTHNFQVDFVWKSTSF DRMQSALKTFAVDETSVSGYIYHKL LGHEVEDVIIKCQLPKRFTAQGLPD LNHSQVYAVKTVLQRPLSLI QGPPGTGKTVTSATIVYHLARQGNG PVLVCAPSNIAVDQLTEKIHQTGLK VVRLCAKSREAIDSPVSFLA LHNQIRNMDSMPELQKLQQLKDETG ELSSADEKRYRALKRTAERELLMNA DVICCTCVGAGDPRLAKMQF RSILIDESTQATEPECMVPVVLGAK QLILVGDHCQLGPVVMCKKAAKAGL SQSLFERLVVLGIRPIRLQV QYRMHPALSAFPSNIFYEGSLQNGV TAADRVKKGFDFQWPQPDKPMFFYV TQGQEEIASSGTSYLNRTEA ANVEKITTKLLKAGAKPDQIGIITP YEGQRSYLVQYMQFSGSLHTKLYQE VEIASVDAFQGREKDFIILS CVRANEHQGIGFLNDPRRLNVALTR ARYGVIIVGNPKALSKQPLWNHLLN YYKEQKVLVEGPLNNLRESL MQFSKPRKLVNTINPGARFMTTAMY DAREAIIPGSVYDRSSQGRPSSMYF QTHDQIGMISAGPSHVAAMN IPIPFNLVMPPMPPPGYFGQANGPA AGRGTPKGKTGRGGRQKNRFGLPGP SQTNLPNSQASQDVASQPFS QGALTQGYISMSQPSQMSQPGLSQP ELSQDSYLGDEFKSQIDVALSQDST YQGERAYQHGGVTGLSQYTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: UPF1 UPF1 RNA helicase and ATPase [ Homo sapiens (human) ]
• Official Symbol: UPF1
• Synonyms: UTF; HUPF1; NORF1; RENT1; smg-2; pNORF1
• Gene ID: 5976
• mRNA Refseq: NM_002911.4
• Protein Refseq: NP_002902.2
• MIM: 601430
• UniProt ID: Q92900