Recombinant Full Length Human VIL1 Protein, C-Flag-tagged

• Cat.No.: VIL1-278HFL
• Product Overview: Recombinant Full Length Human VIL1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 92.5 kDa
• AA Sequence: MTKLSAQVKGSLNITTPGLQIWRIE AMQMVPVPSSTFGSFFDGDCYIILA IHKTASSLSYDIHYWIGQDS SLDEQGAAAIYTTQMDDFLKGRAVQ HREVQGNESEAFRGYFKQGLVIRKG GVASGMKHVETNSYDVQRLL HVKGKRNVVAGEVEMSWKSFNRGDV FLLDLGKLIIQWNGPESTRMERLRG MTLAKEIRDQERGGRTYVGV VDGENELASPKLMEVMNHVLGKRRE LKAAVPDTVVEPALKAALKLYHVSD SEGNLVVREVATRPLTQDLL SHEDCYILDQGGLKIYVWKGKKANE QEKKGAMSHALNFIKAKQYPPSTQV EVQNDGAESAVFQQLFQKWT ASNRTSGLGKTHTVGSVAKVEQVKF DATSMHVKPQVAAQQKMVDDGSGEV QVWRIENLELVPVDSKWLGH FYGGDCYLLLYTYLIGEKQHYLLYV WQGSQASQDEITASAYQAVILDQKY NGEPVQIRVPMGKEPPHLMS IFKGRMVVYQGGTSRTNNLETGPST RLFQVQGTGANNTKAFEVPARANFL NSNDVFVLKTQSCCYLWCGK GCSGDEREMAKMVADTISRTEKQVV VEGQEPANFWMALGGKAPYANTKRL QEENLVITPRLFECSNKTGR FLATEIPDFNQDDLEEDDVFLLDVW DQVFFWIGKHANEEEKKAAATTAQE YLKTHPSGRDPETPIIVVKQ GHEPPTFTGWFLAWDPFKWSNTKSY EDLKAELGNSRDWSQITAEVTSPKV DVFNANSNLSSGPLPIFPLE
  QLVNKPVEELPEGVDPSRKEEHLSI EDFTQAFGMTPAAFSALPRWKQQNL KKEKGLFTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: VIL1 villin 1 [ Homo sapiens (human) ]
• Official Symbol: VIL1
• Synonyms: VIL; D2S1471
• Gene ID: 7429
• mRNA Refseq: NM_007127.3
• Protein Refseq: NP_009058.2
• MIM: 193040
• UniProt ID: P09327