Recombinant Full Length Human WFS1 Protein, C-Flag-tagged

• Cat.No.: WFS1-852HFL
• Product Overview: Recombinant Full Length Human WFS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 100.1 kDa
• AA Sequence: MDSNTAPLGPSCPQPPPAPQPQARS RLNATASLEQERSERPRAPGPQAGP GPGVRDAAAPAEPQAQHTRS RERADGTGPTKGDMEIPFEEVLERA KAGDPKAQTEVGKHYLQLAGDTDEE LNSCTAVDWLVLAAKQGRRE AVKLLRRCLADRRGITSENEREVRQ LSSETDLERAVRKAALVMYWKLNPK KKKQVAVAELLENVGQVNEH DGGAQPGPVPKSLQKQRRMLERLVS SESKNYIALDDFVEITKKYAKGVIP SSLFLQDDEDDDELAGKSPE DLPLRLKVVKYPLHAIMEIKEYLID MASRAGMHWLSTIIPTHHINALIFF FIVSNLTIDFFAFFIPLVIF YLSFISMVICTLKVFQDSKAWENFR TLTDLLLRFEPNLDVEQAEVNFGWN HLEPYAHFLLSVFFVIFSFP IASKDCIPCSELAVITGFFTVTSYL SLSTHAEPYTRRALATEVTAGLLSL LPSMPLNWPYLKVLGQTFIT VPVGHLVVLNVSVPCLLYVYLLYLF FRMAQLRNFKGTYCYLVPYLVCFMW CELSVVILLESTGLGLLRAS IGYFLFLFALPILVAGLALVGVLQF ARWFTSLELTKIAVTVAVCSVPLLL RWWTKASFSVVGMVKSLTRS SMVKLILVWLTAIVLFCWFYVYRSE GMKVYNSTLTWQQYGALCGPRAWKE TNMARTQILCSHLEGHRVTW TGRFKYVRVTDIDNSAESAINMLPF FIGDWMRCLYGEAYPACSPGNTSTA EEELCRLKLLAKHPCHIKKF DRYKFEITVGMPFSSGADGSRSREE DDVTKDIVLRASSEFKSVLLSLRQG SLIEFSTILEGRLGSKWPVF
  ELKAISCLNCMAQLSPTRRHVKIEH DWRSTVHGAVKFAFDFFFFPFLSAA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: WFS1 wolframin ER transmembrane glycoprotein [ Homo sapiens (human) ]
• Official Symbol: WFS1
• Synonyms: WFS; WFRS; WFSL; CTRCT41
• Gene ID: 7466
• mRNA Refseq: NM_006005.3
• Protein Refseq: NP_005996.2
• MIM: 606201
• UniProt ID: O76024