Recombinant Full Length Human WNK4 Protein, C-Flag-tagged

• Cat.No.: WNK4-1157HFL
• Product Overview: Recombinant Full Length Human WNK4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 134.6 kDa
• AA Sequence: MLASPATETTVLMSQTEADLALRPP PPLGTAGQPRLGPPPRRARRFSGKA EPRPRSSRLSRRSSVDLGLL SSWSLPASPAPDPPDPPDSAGPGPA RSPPPSSKEPPEGTWTEGAPVKAAE DSARPELPDSAVGPGSREPL RVPEAVALERRREQEEKEDMETQAV ATSPDGRYLKFDIEIGRGSFKTVYR GLDTDTTVEVAWCELQTRKL SRAERQRFSEEVEMLKGLQHPNIVR FYDSWKSVLRGQVCIVLVTELMTSG TLKTYLRRFREMKPRVLQRW SRQILRGLHFLHSRVPPILHRDLKC DNVFITGPTGSVKIGDLGLATLKRA SFAKSVIGTPEFMAPEMYEE KYDEAVDVYAFGMCMLEMATSEYPY SECQNAAQIYRKVTSGRKPNSFHKV KIPEVKEIIEGCIRTDKNER FTIQDLLAHAFFREERGVHVELAEE DDGEKPGLKLWLRMEDARRGGRPRD NQAIEFLFQLGRDAAEEVAQ EMVALGLVCEADYQPVARAVRERVA AIQRKREKLRKARELEALPPEPGPP PATVPMAPGPPSVFPPEPEE PEADQHQPFLFRHASYSSTTSDCET DGYLSSSGFLDASDPALQPPGGVPS SLAESHLCLPSAFALSIPRS GPGSDFSPGDSYASDAASGLSDVGE GMGQMRRPPGRNLRRRPRSRLRVTS VSDQNDRVVECQLQTHNSKM VTFRFDLDGDSPEEIAAAMVYNEFI LPSERDGFLRRIREIIQRVETLLKR DTGPMEAAEDTLSPQEEPAP LPALPVPLPDPSNEELQSSTSLEHR SWTAFSTSSSSPGTPLSPGNPFSPG TPISPGPIFPITSPPCHPSP SPFSPISSQVSSNPSPHPTSSPLPF SSSTPEFPVPLSQCPWSSLPTTSPP TFSPTCSQVTLSSPFFPPCP STSSFPSTTAAPLLSLASAFSLAVM TVAQSLLSPSPGLLSQSPPAPPSPL PSLPLPPPVAPGGQESPSPH TAEVESEASPPPARPLPGEARLAPI SEEGKPQLVGRFQVTSSKEPAEPLP LQPTSPTLSGSPKPSTPQLT SESSDTEDSAGGGPETREALAESDR AAEGLGAGVEEEGDDGKEPQVGGSP QPLSHPSPVWMNYSYSSLCL SSEESESSGEDEEFWAELQSLRQKH LSEVETLQTLQKKEIEDLYSRLGKQ PPPGIVAPAAMLSSRQRRLS
  KGSFPTSRRNSLQRSEPPGPGIMRR NSLSGSSTGSQEQRASKGVTFAGDV GRMSGPTRTRRLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: WNK4 WNK lysine deficient protein kinase 4 [ Homo sapiens (human) ]
• Official Symbol: WNK4
• Synonyms: PHA2B; PRKWNK4
• Gene ID: 65266
• mRNA Refseq: NM_032387.5
• Protein Refseq: NP_115763.2
• MIM: 601844
• UniProt ID: Q96J92