Recombinant Full Length Human ZBTB33 Protein, GST-tagged

• Cat.No.: ZBTB33-6851HF
• Product Overview: Human ZBTB33 full-length ORF ( AAH42753.1, 1 a.a. - 673 a.a.) recombinant protein with GST-tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 101 kDa
• Protein Length: 673 amino acids
• AA Sequence: MESRKLISATDIQYSGSLLNSLNEQ RGHGLFCDVTVIVEDRKFRAHKNIL SASSTYFHQLFSVAGQVVELSFIRA EIFAEILNYIYSSKIVRVRSDLLDE LIKSGQLLGVKFIAELGVPLSQVKS ISGTAQDGNTEPLPPDSGDKNLVIQ KSKDEAQDNGATIMPIITESFSLSA EDYEMKKIIVTDSDDDDDDDVIFCS EILPTKETLPSNNTVAQVQSNPGPV AISDVAPSASNNSPPLTNITPTQKL PTPVNQATLSQTQGSEKLLVSSAPT HLTPNIILLNQTPLSTPPNVSSSLP NHMPSSINLLVQNQQTPNSAILTGN KANEEEEEEIIDDDDDTISSSPDSA VSNTSLVPQADTSQNTSFDGSLIQK MQIPTLLQEPLSNSLKISDIITRNT NDPGVGSKHLMEGQKIITLDTATEI EGLSTGCKVYANIGEDTYDIVIPVK DDPDEGEARLENEIPKTSGSEMANK RMKVKHDDHYELIVDGRVYYICIVC KRSYVCLTSLRRHFNIHSWEKKYPC RYCEKVFPLAEYRTKHEIHHTGERR YQCLACGKSFINYQFMSSHIKSVHS QDPSGDSKLYRLHPCRSLQIRQYAY LSDRSSTIPAMKDDGIGYKVDTGKE PPVGTTTSTQNKPMTWEDIFIQQEN DSIFKQNVTDGSTEFEFIIPESY
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ZBTB33 zinc finger and BTB domain containing 33 [ Homo sapiens (human) ]
• Official Symbol: ZBTB33
• Synonyms: ZBTB33; zinc finger and BTB domain containing 33; ZNF348; ZNF-kaiso; transcriptional regulator Kaiso; UGSC:H_DJ525N14.1; kaiso transcription factor; zinc finger and BTB domain-containing protein 33
• Gene ID: 10009
• mRNA Refseq: NM_006777
• Protein Refseq: NP_006768
• MIM: 300329
• UniProt ID: Q86T24