Recombinant Full Length Mycobacterium tuberculosis CYP51 Protein, His tagged

• Cat.No.: CYP51-01M
• Product Overview: CYP51 Protein, Mycobacterium tuberculosis (sf9, His) is the recombinant CYP51 protein, expressed by Sf9 insect cells, with N-10*His labeled tag.

Specification

• Species: Mycobacterium tuberculosis
• Source: Insect Cells
• Tag: His
• Protein Length: 1-451 aa
• Description: Sterol 14alpha-demethylase whose physiological substrate is not known. Accepts electrons from the iron-sulfur ferredoxin Fdx encoded by an adjacent gene. In vitro, catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol, to produce the 8,14-dienes stereoselectively.
• Form: Lyophilized powder. Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
• Bio-activity: The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
• Molecular Mass: 54 kDa
• AASequence: MSAVALPRVSGGHDEHGHLEEFRTDPIGLMQRVRDECGDVGTFQLAGKQVVLLSGSHANEFFFRAGDDDLDQAKAYPFMTPIFGEGVVFDASPERRKEMLHNAALRGEQMKGHAATIEDQVRRMIADWGEAGEIDLLDFFAELTIYTSSACLIGKKFRDQLDGRFAKLYHELERGTDPLAYVDPYLPIESFRRRDEARNGLVALVADIMNGRIANPPTDKSDRDMLDVLIAVKAETGTPRFSADEITGMFISMMFAGHHTSSGTASWTLIELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLRLHPPLIILMRVAKGEFEVQGHRIHEGDLVAASPAISNRIPEDFPDPHDFVPARYEQPRQEDLLNRWTWIPFGAGRHRCVGAAFAIMQIKAIFSVLLREYEFEMAQPPESYRNDHSKMVVQLAQPACVRYRRRTGV
• Endotoxin: <1 EU/μg, determined by LAL method.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Stored at -20 centigrade for 2 years from date of receipt. After reconstitution, it is stable at 4 centigrade for 1 week or -20 centigrade for longer (with carrier protein). It is recommended to freeze aliquots at -20 or -80 centigrade for extended storage.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Gene Information

• Gene Name: cyp51 lanosterol 14-alpha demethylase [ Mycobacterium tuberculosis H37Rv ]
• Official Symbol: cyp51
• Synonyms: cyp51; lanosterol 14-alpha demethylase; lanosterol 14-alpha demethylase; Cytochrome P450 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM); Sterol 14alpha-demethylase; EC:1.14.15.36; CYPLI; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; Sterol 14-alpha demethylase
• Gene ID: 888819
• Protein Refseq: NP_215278
• UniProt ID: P9WPP8