Recombinant Full Length Thermoanaerobacter Thermosulfurogenes Probable Starch Degradation Products Transport System Permease Protein Amyd(Amyd) Protein, His-Tagged

• Cat.No.: RFL12336TF
• Product Overview: Recombinant Full Length Thermoanaerobacter thermosulfurogenes Probable starch degradation products transport system permease protein AmyD(amyD) Protein (P37730) (1-292aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Thermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-292)
• AA Sequence: MAKKAKFFKNGIWYWLFIAPTLLSL IIVVLIPFIIGIYYSFTDWNGINQP VFIGLKNFMT LRDDAEFWNSIIFTAKFAVACIVII NVVGLSLAMLVTRKIFARNFMRTAF YLPNLIGGLI LGFIWNFIFVDVFQTISDATHIGWL GGWLSTTNTGFWGLVIVTSWQMIGY VMVIYIAYIE SIPTDLIEASKIDGANSWQQFRNVV FPLIAPAFTVSLFITLSNSFKLFDQ NLSLTAGAPG NTTQMITLNIYQTAFSAQEMAVGQA KAVIMFLIIAVISVIQVYLTQKREV EM
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: amyD
• Synonyms: amyD; Probable starch degradation products transport system permease protein AmyD
• UniProt ID: P37730

Gene Information

• Gene Name: amyD
• Synonyms: amyD; Probable starch degradation products transport system permease protein AmyD
• UniProt ID: P37730

Reviews

09/14/2022

With their meticulous documentation and support, I am confident that my utilization of the amyD protein in clinical trials will meet all necessary regulations and guidelines.

03/24/2019

The amyD protein offered by the manufacturer excels in its quality and is an excellent match for my experimental requirements.

05/19/2018

Their ability to accommodate the requirements of large-scale experiments and guarantee a steady supply streamlines my research operations, eliminating any concerns related to prospective shortages.

02/19/2018

Their team of experts is readily available to address any concerns or queries I may have regarding the amyD protein.

07/17/2017

Their comprehensive certificates of analysis and safety data sheets serve as testaments to their commitment to ensuring the safety and integrity of their products.

Q&As

Can the amyD protein be used in food processing or industrial applications?

Yes, the amyD protein and other amylases are widely used in food processing and various industrial applications. In food processing, amylases are used for starch hydrolysis, which helps break down complex carbohydrates into simpler sugars. This is important in processes such as baking, brewing, and the production of various food products. Amylases are also used in the production of sweeteners like glucose syrup or high fructose corn syrup.

Is the production of the amyD protein regulated?

Yes, the production of the amyD protein is regulated by various factors, including the presence of starch or other carbohydrate sources in the environment. When starch is available, certain signaling pathways or genetic mechanisms can be activated to induce the expression of the amyD gene and subsequent production of the amyD protein.

Are there any genetic variations or polymorphisms of the amyD gene?

Genetic variations or polymorphisms in the amyD gene can occur, leading to differences in the amino acid sequence of the amyD protein. These variations can influence the stability, activity, and regulation of the protein. Genetic variations in amylase genes have been of interest in human populations, as some populations have a higher number of amylase gene copies, associated with increased amylase production and enhanced starch digestion.

Are there any pharmaceutical drugs or inhibitors that target the amyD protein?

While there are no specific pharmaceutical drugs that directly target the amyD protein, there are inhibitors that can affect the activity of amylases in general. These inhibitors can be utilized in certain medical conditions where inhibition of amylase activity is desired, such as in the management of hyperamylasemia or acute pancreatitis. Examples of such inhibitors include acarbose, which is used to treat diabetes, and competitive inhibitors that can selectively inhibit amylase activity.

What are the structural features of the amyD protein?

The amyD protein generally possesses a domain structure that is characteristic of amylases. It typically consists of a catalytic domain, which is responsible for the enzymatic activity of breaking down starch, and sometimes additional domains for substrate binding or other functions. The specific structural features can vary between different species or strains that produce the amyD protein.

Are there any other functions or roles of the amyD protein besides carbohydrate digestion?

While the main function of the amyD protein is carbohydrate digestion, certain studies have suggested that it may also play a role in other processes. For example, in some bacteria, the amyD protein has been implicated in biofilm formation, which is a structured community of microorganisms attached to a surface. Additionally, the amyD protein has been found to be involved in the production of certain secondary metabolites in some fungi.

Are there any diseases or disorders associated with the amyD protein?

There are no known diseases or disorders specifically associated with the amyD protein itself. However, dysregulation or malfunction of the amylase enzymes, including the amyD protein, can lead to carbohydrate metabolism disorders, such as amylase deficiency. Additionally, excessive production or activity of amylases can be associated with certain pathological conditions, such as pancreatic disorders or salivary gland abnormalities.

Are there any known natural inhibitors or activators of the amyD protein?

There are several factors that can affect the activity of the amyD protein. For example, the presence of certain ions, such as calcium or chloride, can enhance the activity of amylases, including amyD. On the other hand, some chemicals or compounds, such as certain detergents or heavy metals, can act as inhibitors and reduce the activity of amylases. Additionally, pH and temperature can also impact the activity of amylases, with optimal conditions varying depending on the specific enzyme and microorganism.

How does the amyD protein function in carbohydrate digestion?

The amyD protein acts as an enzyme and catalyzes the hydrolysis of starch molecules. It breaks down the long chains of starch into smaller sugar molecules, such as maltose and glucose. This process is crucial for the absorption of carbohydrates during digestion.

Is the amyD protein similar to other amylases in terms of its structure and function?

Yes, the amyD protein is part of the amylase family and shares structural and functional similarities with other amylases. These similarities include having a similar catalytic mechanism for starch hydrolysis and possessing conserved regions and domains that are characteristic of amylases. However, the amyD protein may have specific variations or adaptations that differentiate it from other amylases, depending on the species or strain that produces it.

Can the amyD protein be used in industrial or biotechnological applications?

Yes, the amyD protein and other amylases have been widely used in various industrial and biotechnological applications. Their ability to efficiently degrade starch and convert it into useful sugars makes them valuable for processes such as starch hydrolysis for biofuel production, food processing, brewing, and several other applications in the agricultural and pharmaceutical industries.

Can the amyD protein be used in genetic engineering or biotechnology applications?

Yes, the amyD protein and other amylases can be used in genetic engineering and biotechnology applications. For example, the amyD gene can be cloned and expressed in other organisms to enable starch digestion and utilization in those organisms. This can be useful for bioremediation purposes or for production of valuable products from starch substrates.