Recombinant Green Fluorescent Protein, His tagged, Protein G Labeled

• Cat.No.: GFP-05
• Product Overview: Protein G labeled recombinant Green Fluorescent Protein with His tag was expressed in E. coli.

Specification

• Source: E.coli
• Tag: His
• Conjugation/Label: Protein G
• Description: Antibody Binding GFP is a Protein G-GFP fusion protein. It consists of protein G and enhanced green fluorescent protein (EGFP), while the two proteins connect via a short peptide. Protein G tightly binds to IgG molecules from rabbit, human, mouse, goat, sheep, cow, and rat. Since protein A binds poorly to human IgG3, mouse IgG1, and immunoglobulins from rat, sheep, and horse, protein G conjugates are especially valuable for detecting these antibodies. EGFP has an excitation and emission spectra of 488/509nm, allowing its detection under fluorescent microscopy and flow cytometry. As an antibody binding GFP, Protein G-EGFP protein can avoid harmful labeling of the detecting antibody. Because staining protocols vary with application, the appropriate dilution of Antibody Binding GFP should be determined empirically.
• Form: Liquid
• Molecular Mass: 51.6 kDa
• Purity: ≥ 90% by SDS-PAGE
• Applications: In vitro research use only
• Storage: Short Term Storage: -20 centigrade Long Term Storage: -20 centigrade Avoid freeze/thaw cycle. Aliquot upon arrival.
• Storage Buffer: 50 mM sodium phosphate,100 mM NaCl, 1 mM DTT, pH7.2, 50% glycerol
• Concentration: 0.5-1.0 mg/mL
• Shipping: Cold packs

Gene Information

• Official Symbol: GFP
• Synonyms: GFP; Green Fluorescent Protein