Recombinant H10N9 (A/duck/Hong Kong/562/1979) HA Protein, His-tagged

• Cat.No.: HA-574V
• Product Overview: Recombinant N-terminal segment (Met1-Asp524) of the influenza hemagglutinin (A/duck/Hong Kong/562/1979 (H10N9)) (ABI84469.1), termed as HA, was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: H10N9
• Source: HEK293
• Tag: His
• Protein Length: 1-524 a.a.
• Predicted N Terminal: Asp 18
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant HA subunit of influenza A H12N1 HA (A/duck/HongKong/562/1979(H10N9)) comprises 519 amino acids and has a predicted molecular mass of 57.7 kDa. As a result of glycosylation, it migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >87 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.