Recombinant H12N5 (A/green-winged teal/ALB/199/1991) HA Protein, His-tagged

• Cat.No.: HA-408V
• Product Overview: Recombinant H12N5 (A/green-winged teal/ALB/199/1991) HA Protein(Met 1-Lys 527)(ABB88110.1) (HA1+HA2,uncleaved) was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Species: H12N5
• Source: HEK293
• Tag: His
• Protein Length: 1-527 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant influenza A H12N5 HA (A/green-winged teal/ALB/199/1991 (H12N5)) comprises 521 amino acids and has a predicted molecular mass of 59.3 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.