Recombinant H1N1(A/Brevig Mission/1/1918) HA1 Subunit protein, His-tagged

• Cat.No.: HA-7843V
• Product Overview: Recombinant H1N1(A/Brevig Mission/1/1918) HA1 Subunit protein(AAD17229.1) (Met 1-Arg 344), termed as HA1 subunit, was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: H1N1
• Source: HEK293
• Tag: His
• Protein Length: 1-344 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: Recombinant HA1 subunit of Influenza A virus H1N1 hemagglutinin (A/Brevig Mission/1/1918 (H1N1)) comprises 338 amino acids with the predicted molecular mass of 37.5 kDa. As a result of glycosylation, it migrates as an approximately 53 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.