Recombinant H1N1 (A/California/04/2009) HA protein, His-tagged

• Cat.No.: HA-2664V
• Product Overview: Recombinant H1N1 (A/California/04/2009) HA protein(ACP41105.1) (Met 1-Gln 529) with cleavage site mutated (IQS→RQRRRRK, HA1+HA2, partially cleaved) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: H1N1
• Source: HEK293
• Tag: His
• Protein Length: 1-529 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant Influenza A virus (A/California/04/2009 (H1N1)) HA (mutated) comprises 527 amino acids and predicts a molecular mass of 60 kDa. As a result of glycosylation and proteolysis, the apparent molecular mass of the hemagglutinin is apparoximately 25-30, 50-55 and 80-85 kDa in SDS-PAGE under reducing conditions, corresponding to the HA2, HA1 subunit and the full length HA respectively.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.