Recombinant H1N1 (A/Puerto Rico/8/34) HA Protein, His-tagged

• Cat.No.: HA-333V
• Product Overview: Recombinant H1N1 (A/Puerto Rico/8/34) HA Protein(Met 1-Gln 528)(ABD77675.1) was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Species: H1N1
• Source: HEK293
• Tag: His
• Protein Length: 1-528 aa
• Form: Supplied as sterile 20mM Tris,150mM NaCl,0.1M Arg,0.1M Glu,10%glycerol,0.01% tween20, pH8.5.
• Molecular Mass: The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/ml