Recombinant H1N1(A/California/04/2009) NA protein, Fc-tagged

• Cat.No.: NA-777H
• Product Overview: Recombinant H1N1(A/California/04/2009) NA protein(ACP41107.1) (His 36-Lys 469) was expressed in HEK293 with the fused Fc region of human IgG1 at the N-terminus linked by a peptide linker.

Specification

• Species: H1N1
• Source: HEK293
• Tag: Fc
• Protein Length: 36-469 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant neuraminidase (A/California/04/2009 (H1N1))/Fc chimera is a disulfied-lined homodimer. The reduced monomer comprises 692 amino acids with the predicted molecular mass of 76 kDa. As a result of glycosylation, it migrates as an approximately 95-105 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.