Recombinant H3N2 (A/Brisbane/10/2007) HA Protein, His-tagged

• Cat.No.: HA-2931V
• Product Overview: Recombinant H3N2 (A/Brisbane/10/2007) HA Protein(ABW23353.1)(Met 1-Ile 531) (Native, HA1+HA2, uncleaved) was expressed in HEK293 with a C-terminal polyhistidine.

Specification

• Species: H3N2
• Source: HEK293
• Tag: His
• Protein Length: 1-531 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/Brisbane/10/2007 (H3N2)) comprises 526 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 90-100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.