Recombinant H5N1 (A/Anhui/1/2005) HA Protein, His-tagged

• Cat.No.: HA-273V
• Product Overview: Recombinant extracellular domain of Influenza A virus (A/Anhui/1/2005(H5N1) (ABD28180.1) hemagglutinin (Met 1-Gln 527,Ala 150Val) with cleavage site mutated (RERRRKR→TETR, HA1+HA2, uncleaved) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.

Specification

• Species: H5N1
• Source: Insect Cells
• Tag: His
• Protein Length: 1-527 a.a.
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile 20mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/Anhui/1/ 2005(H5N1) comprises 557 amino acids with the predicted molecular mass of 63 kDa. As a result of glycosylation, it migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.This recombinant HA proteins are polymers based on HPLC analysis (data not shown).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.