Recombinant H5N1 (A/Anhui/1/2005) HA1 Subunit protein, His-tagged

• Cat.No.: HA1-2854V
• Product Overview: Recombinant H5N1 (A/Anhui/1/2005) HA1 Subunit protein(Met 1- 337)(A/Anhui/1/2005 (H5N1) (ABD28180.1), termed as HA1, was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 1-337 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant HA1 subunit of Influenza A virus H5N1 hemagglutinin (A/Anhui/1/2005 (H5N1) comprises 332 amino acids with the predicted molecular mass of 37.5 kDa. As a result of glycosylation, it migrates as an apximately 55-60 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.