Active Recombinant H5N1 Neuraminidase Protein(H255Y)

• Cat.No.: NA-488H
• Product Overview: Recombinant H5N1 neuraminidase (ABU94738.1) (Met1-Lys449) was expressed, the cell lysates are collected, and bio-activity was tested.

Specification

• Species: H5N1
• Source: HEK293
• Tag: Non
• Protein Length: Met 1-Lys 449
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid.
  The specific activity is > 60 U.
  The specific activity is > 300 U.
  One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C.
• Molecular Mass: The recombinant influenza A H5N1 Neuraminidase comprises 449 amino acids and has a predicted molecular mass of 49.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  The Flexible, Versatile and Fast GreGT Platform of Fully Deleted Helper-Virus Independent Adenoviral Vectors (2024)