| Species : |
H7N9 |
| Source : |
Insect Cells |
| Tag : |
His |
| Protein Length : |
1-524 a.a. |
| Form : |
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.5 μg/mL for 1% GRBC. It also agglutinates chick red blood cells. 2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. |
| Molecular Mass : |
The recombinant hemagglutinin of Influenza A virus (A/Shanghai/1/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.7 kDa. The apparent molecular mass of the protein is approximately 55-59 kDa in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 90 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
