Recombinant HCoV-EMC/2012 N, His tagged

• Cat.No.: NP-530H
• Product Overview: Recombinant HCoV-EMC/2012 N (AFS88943.1) (Met1-Asp413), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: HCoV-EMC/2012
• Source: Insect Cells
• Tag: His
• Protein Length: 1-413 a.a.
• Predicted N Terminal: Met 1
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant Nucleoprotein (Human betacoronavirus 2c EMC/2012) comprises 423 amino acids and has a predicted molecular mass of 46.4 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: N?N protein [?Middle East respiratory syndrome coronavirus?]
• Official Symbol: N
• Gene ID: 14254601
• Protein Refseq: YP_009047211
• UniProt ID: AFS88943