Optional Service: Optional requirements on this protein
Product Overview :
HCoV-NL63 S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 19 - Val 717 (Accession # Q6Q1S2-1).
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass :
The protein has a calculated MW of 79.5 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
Protein length :
Cys 19 - Val 717
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.