Recombinant HCoV-NL63 Spike Trimer (S1+S2), C-His-tagged

• Cat.No.: Spike-02H
• Product Overview: Human Coronavirus NL63 Spike trimer (S1+S2) (HCoV-NL63 S protein), Genbank Accession No. APF29071, a.a. 1-1296(full length), with a C-terminal His-tag, expressed in a HEK293 expression system. MW=146 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Specification

• Species: HCoV-NL63
• Source: HEK293
• Tag: His
• Protein Length: 1-1296 a.a.
• Description: S1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
• Form: Aqueous buffer solution.
• Molecular Mass: 146 kDa
• AA Sequence: 1-1296(full length)
• Purity: >90%
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: At least 6 months at -80 centigrade.
• Storage Buffer: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
• Shipping: -80 centigrade
• Warning: Avoid freeze/thaw cycles.
• Full Length: Full L.

Gene Information

• Official Symbol: S
• Synonyms: S; Spike glycoprotein; S glycoprotein; E2; Peplomer protein
• UniProt ID: A0A1L2YVI8