|Product Overview:||The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci.|
|Description:||Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.|
|Form:||1 mg/ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration|
|Bio-activity:||The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.|
|Purity:||Over 98% by SDS-PAGE|
|Unit Definition:||Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37centigrade for 30 min.|
|Applications:||1).Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent).
2).Reagent for membrane pore formation to introduce small-to-macromolecules into living cells.
|Notes:||Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT.|
|Storage:||Store at -20 centigrade(long period, -70 centigrade).|
|Warning:||Handling and storage precautions: Biohazard. Do not use if skin is cut or scratched.
Other precautions: Substance not yet fully tested.
Protective gloves: compatible chemical-resistant gloves.
Eye protection: ansi approved chemical workers goggle.
Other protective equipment: eye wash and deluge shower meeting ansi design criteria. Work hygienic practices: wash thoroughly after handling.
|Gene Name:||Streptolysin O precursor [Streptococcus pyogenes M1 GAS]|
|Synonyms:||Streptolysin O; slo|
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