Active Recombinant Hemolytic streptococcus Streptolysin O

• Cat.No.: slo-001S
• Product Overview: The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci.

Specification

• Description: Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
• Source: E. coli
• Species: Hemolytic streptococcus
• Form: 1 mg/ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration
• Bio-activity: The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.
• AA Sequence: NKQNTANTETTTTNEQPKPESSELT TEKAGQKMDDMLNSNDMIKLAPKEM PLESAEKEEKKSEDNKKSEEDHTEE INDKIYSLNYNELEVLAKNGETIEN FVPKEGVKKADKFIVIERKKKNINT TPVDISIIDSVTDRTYPAALQLANK GFTENKPDAVVTKRNPQKIHIDLPG MGDKATVEVNDPTYANVSTAIDNLV NQWHDNYSGGNTLPARTQYTESMVY SKSQIEAALNVNSKILDGTLGIDFK SISKGEKKVMIAAYKQIFYTVSANL PNNPADVFDKSVTLKELQRKGVSNE APPLFVSNVAYGRTVFVKLETSSKS NDVEAAFSAALKGTDVKTNGKYSDI LENSSFTAVVLGGDAAEHNKVVTKD FDVIRNVIKDNATFSRKNPAYPISY TSVFLKNNKIAGVNNRSEYVETTST EYTSGKINLSHQGAYVAQYEILWDE INYDDKGKEVITKRRWDNNWYSKTS PFSTVIPLGANSRNIRIMARECTGL AWEWWRKVIDERDVKLSKEINVNIS GSTLSPYGSITYK
• Purity: Over 98% by SDS-PAGE
• Unit Definition: Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37centigrade for 30 min.
• Applications: 1).Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent).
  2).Reagent for membrane pore formation to introduce small-to-macromolecules into living cells.
• Notes: Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT.
• Storage: Store at -20 centigrade(long period, -70 centigrade).
• Warning: Handling and storage precautions: Biohazard. Do not use if skin is cut or scratched.
  Other precautions: Substance not yet fully tested.
  Protective gloves: compatible chemical-resistant gloves.
  Eye protection: ansi approved chemical workers goggle.
  Other protective equipment: eye wash and deluge shower meeting ansi design criteria. Work hygienic practices: wash thoroughly after handling.

Gene Information

• Gene Name: Streptolysin O precursor [Streptococcus pyogenes M1 GAS]
• Official Symbol: slo
• Synonyms: Streptolysin O; slo
• Gene ID: 900490
• Protein Refseq: NP_268546.1
• UniProt ID: Q54114