Recombinant Hepatitis C Virus Core/NS3/NS4/NS5 Protein

• Cat.No.: HCVcAg-343H
• Product Overview: Recombinant Hepatitis C Virus Core/NS3/NS4/NS5 Antigen Protein is produced by E. coli expression system. Molecular weight is 33 kDa measured by SDS-PAGE (includes fusion protein). Contains four antigens expressed in E. coli in a chimera:
  -HCV Core Fragment (a.a. 11-45)
  -HCV NS3 Fragment (a.a. 1208-1430)
  -HCV NS4 Fragment (a.a. 1885-1923)
  -HCV NS5 Fragment (a.a. 2332-2423)

Specification

• Description: Hepatitis C virus (HCV) is an infectious disease that primarily affects the liver. It is the most serious type of hepatitis virus and can lead to cirrhosis and liver failure or cancer. It is estimated that 150–200 million people, or about 3% of the world’s population, are living with chronic HCV.
• Source: E. coli
• Species: HCV
• Form: 20 mM Phosphate Buffer, pH 7.4
• Molecular Mass: 33 kDa
• Purity: > 90% pure (SDS-PAGE).
• Applications: Suitable for use in ELISA and Lateral Flow Double Antigen Sandwich Assay. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
• Storage: Store < 2 weeks at 2-8 centigrade. Long term store at -20 centigrade. Aliquot to avoid multiple freeze/thaw cycles
• Concentration: 5.15 mg/mL ({OD280*1.45} – {OD260*0.74})