Recombinant Hepatitis C virus (HCV) NS3 Protein

• Cat.No.: NS3-36H
• Product Overview: This recombinant protein contains immunodominant regions of HCV NS3 protein with a MW of 70 kDa. It is recognized by positive samples in ELISA tests.

Specification

• Description: HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
• Source: E. coli
• Species: HCV
• Form: Affinity purified, liquid
• Molecular Mass: 70 kDa
• Purity: >95% pure by SDS-PAGE
• Applications: Suitable for use in ELISA, Lateral Flow, WB, and other immune tests.
• Storage: Upon receipt, quick spin before open vial, aliquot and store at ≤ -20 centigrade. Avoid multiple freeze/thaw cycles
• Storage Buffer: Tris-buffered saline, pH 8.0
• Preservative: 0.1% ProClin 300
• Synonyms: NS3; HCV NS3; Hepatitis C Virus NS3

Gene Information

• Synonyms: NS3; HCV NS3; Hepatitis C Virus NS3