Recombinant HIV-1(group M, subtype C, strain 92BR025) Gag protein(Pro133-Leu363), His-tagged

• Cat.No.: Gag-1116V
• Product Overview: Recombinant HIV1 Gag-P24 (subtype C) (AAB61122.1) (Pro133-Leu363) was expressed in E. coli with a polyhistidine tag at the C-terminus.

Specification

• Species: HIV
• Source: E.coli
• Tag: His
• Protein Length: Pro133-Leu363
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant HIV1 Gag-P24 (subtype C) consists of 238 amino acids and predicts a molecular mass of 26.5 kDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.