Recombinant HIV(group M, subtype CRF07_BC) GP120 protein, His-tagged

• Cat.No.: GP120-2220H
• Product Overview: Recombinant HIV(group M, subtype CRF07_BC) GP120 protein(AFM77980.1)(Ala 29-Glu 503) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• Protein Length: 29-503 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant HIV-1 gp120 consists of 486 amino acids after removal of the signal peptide and has a predicted molecular mass of 54.6 kDa. The apparent molecular mass of the recombinant protein is approximately 80-100 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.