| Description : |
Attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. Acts as a ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses. These interactions allow capture of viral particles at mucosal surfaces by these cells and subsequent transmission to permissive cells. HIV subverts the migration properties of dendritic cells to gain access to CD4+ T-cells in lymph nodes. Virus transmission to permissive T-cells occurs either in trans (without DCs infection, through viral capture and transmission), or in cis (following DCs productive infection, through the usual CD4-gp120 interaction), thereby inducing a robust infection. In trans infection, bound virions remain infectious over days and it is proposed that they are not degraded, but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the viral infectious potential during DCs' migration from the periphery to the lymphoid tissues. On arrival at lymphoid tissues, intact virions recycle back to DCs' cell surface allowing virus transmission to CD4+ T-cells. |
| Source : |
HEK293 |
| Species : |
HIV1 |
| Tag : |
His |
| Form : |
Lyophilized |
| Molecular Mass : |
53.8 kDa |
| Protein Length : |
864 |
| AA Sequence : |
MRVKETQMNWPNLWKWGTLILGLVI ICSASDNLWVTVYYGVPVWRDADTT LFCASDAKAHETEVHNVWATHACVP TDPNPQEIDLENVTENFNMWKNNMV EQMQEDVISLWDQSLKPCVKLTPLC VTLHCTNANLTKANLTNVNNRTNVS NIIGNITDEVRNCSFNMTTELRDKK QKVHALFYKLDIVPIEDNNDNSKYR LINCNTSVIKQACPKISFDPIPIHY CTPAGYAILKCNDKNFNGTGPCKNV SSVQCTHGIKPVVSTQLLLNGSLAE EEIIIRSEDLTNNAKTIIVHLNKSV VINCTRPSNNTRTSITIGPGQVFYR TGDIIGDIRKAYCEINGTEWNKALK QVTEKLKEHFNNKPIIFQPPSGGDL EITMHHFNCRGEFFYCNTTRLFNNT CIANGTIEGCNGNITLPCKIKQIIN MWQGAGQAMYAPPISGTINCVSNIT GILLTRDGGATNNTNNETFRPGGGN IKDNWRNELYKYKVVQIEPLGVAPT RAKRRVVEREKRAVGIGAMIFGFLG AAGSTMGAASITLTVQARQLLSGIV QQQSNLLRAIEAQQHLLQLTVWGIK QLQARVLAVERYLKDQKFLGLWGCS GKIICTTAVPWNSTWSNKSLEEIWN NMTWIEWEREISNYTNQIYEILTKS QDQQDRNEKDLLELDKWASLWTWFD ITNWLWYIKIFIMIVGGLIGLRIIF AVLSIVNRVRQGYSPLSFQTPCHHQ REPDRPERIEEEGGEQGRDRSVRLV SGFLALAWDDLRSLCLFSYHRLRDF ILIAARTVELLGRSSLKGLRRGWEG LKYLGNLLLYWGQELKISAISLLDA TAIAVAGWTDRVIEVAQGAWKAILH IPRRIRQGLERALQ |
| Purity : |
> 98% |
| Applications : |
WB; ELISA; FACS; FC |
| Stability : |
This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use. |
| Storage : |
At -20 centigrade. |
| Concentration : |
1 mg/mL |
| Storage Buffer : |
PBS (pH 7.4-7.5). Sterile-filtered colorless solution. |
| Reconstitution : |
Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance. |
