Recombinant HSV 2(strain 333) gB protein(Glu98-Ala730), His-tagged

• Cat.No.: gB-500V
• Product Overview: Recombinant HSV 2(strain 333) gB protein(Glu98-Ala730)(P06763) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: HSV2
• Source: HEK293
• Tag: His
• Protein Length: Glu98-Ala730
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized HSV-2 (strain 333) Glycoprotein B (gB), His Tag at 1 μg/mL (100 μL/well) can bind Mouse anti-Herpes virus 1 and2,clone T111(Monoclonal), Mouse IgG1) with a linear range of 0.06-0.5 ng/mL.
• Molecular Mass: The protein has a calculated MW of 74.3 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.