Recombinant Human 3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1, His-tagged

• Cat.No.: PAPSS1-1390H
• Product Overview: Recombinant humanPAPSS1 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PAPSS1, also knownas bifunctional 3"-phosphoadenosine 5"-phosphosulfate synthase 1, is abifunctional enzyme with both ATP sulfurylase and APS kinase activity, whichmediates two steps in the sulfate activation pathway, yielding3"-phosphoadenylylsulfate (PAPS).
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl.
• Molecular Weight: 70.9 kDa(626aa)
• Purity: > 85%by SDS - PAGE
• Concentration: 0.5mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMRATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLTGLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCITSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAPELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQVLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAILRNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRLTPTELKQKFKDMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEEGVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTMAPGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYYKSLEKA
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PAPSS1 3"-phosphoadenosine5"-phosphosulfate synthase 1 [ Homo sapiens ]
• Official Symbol: PAPSS1
• Synonyms: PAPSS1; 3"-phosphoadenosine5"-phosphosulfate synthase 1; asSK1; PAPSS; ATPSK1; bifunctional3"-phosphoadenosine 5"-phosphosulfate synthase 1; SK 1; PAPSS 1; PAPS synthase1; OTTHUMP00000161864; sulfurylase kinase 1; 3-prime-phosphoadenosine5-prime-phosphosulfate synthase 1; EC 2.7.1.25; EC 2.7.7.4
• Gene ID: 9061
• mRNA Refseq: NM_005443
• Protein Refseq: NP_005434
• MIM: 603262
• UniProt ID: O43252
• Chromosome Location: 4q24
• Pathway: Biologicaloxidations; Cytosolic sulfonation of small molecules; Formation of PAPS;Metabolic pathways; Phase II conjugation; Purine metabolism; Selenocompoundmetabolism; Sulfur metabolism
• Function: ATPbinding; adenylylsulfate kinase activity; nucleotide binding; nucleotidyltransferaseactivity; sulfate adenylyltransferase (ATP) activity