Recombinant Human 4-Hydroxyphenylpyruvate Dioxygenase, His-tagged

• Cat.No.: HPD-1705H
• Product Overview: Recombinant human HPD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: 4-hydroxyphenylpyruvate dioxygenase isoform 1, also known as HPD, is an Fe-containing enzyme, that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, HPD uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding HPD are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities.
• Concentration: 1 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 50mM NaCl.
• Purity: > 90% by SDS - PAGE
• Sequence: 1-393 amino acids: MGSSHHHHHH SSGLVPRGSH MTTYSDKGAK PERGRFLHFH SVTFWVGNAK QAASFYCSKM GFEPLAYRGL ETGSREVVSH VIKQGKIVFV LSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIMRE PWVEQDKFGK VKFAVLQTYG DTTHTLVEKM NYIGQFLPGY EAPAFMDPLL PKLPKCSLEM IDHIVGNQPD QEMV
• Molecular Mass: 47 kDa (413aa) confirmed by MALDI-TOF
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HPD 4-hydroxyphenylpyruvate dioxygenase [ Homo sapiens ]
• Official Symbol: HPD
• Synonyms: HPD; 4-hydroxyphenylpyruvate dioxygenase; PPD; 4HPPD; GLOD3; 4-HPPD; HPPDASE; glyoxalase domain containing 3; 4-hydroxyphenylpyruvic acid oxidase; EC 1.13.11.27
• Gene ID: 3242
• mRNA Refseq: NM_001171993
• Protein Refseq: NP_001165464
• MIM: 609695
• UniProt ID: P32754
• Chromosome Location: 12q24-qter
• Pathway: Metabolic pathways; Phenylalanine metabolism; Tyrosine metabolism; Ubiquinone and other terpenoid-quinone biosynthesis
• Function: 4-hydroxyphenylpyruvate dioxygenase activity; metal ion binding; oxidoreductase activity