Description : |
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. |
Source : |
E. coli |
Species : |
Human |
Tag : |
N-His |
Form : |
Freeze-dried powder |
Molecular Mass : |
Predicted Molecular Mass: 19.4 kDa |
Protein length : |
Pro1185-Phe1352 |
Purity : |
> 97% |
Applications : |
Positive Control; Immunogen; SDS-PAGE; WB. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
Storage Buffer : |
PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300. |
Reconstitution : |
Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex. |