| Description : |
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
120.8 kDa |
| AA Sequence : |
MSAKAISEQTGKELLYKFICTTSAI QNRFKYARVTPDTDWARLLQDHPWL LSQNLVVKPDQLIKRRGKLGLVGVN LTLDGVKSWLKPRLGQEATVGKATG FLKNFLIEPFVPHSQAEEFYVCIYA TREGDYVLFHHEGGVDVGDVDAKAQ KLLVGVDEKLNPEDIKKHLLVHAPE DKKEILASFISGLFNFYEDLYFTYL EINPLVVTKDGVYVLDLAAKVDATA DYICKVKWGDIEFPPPFGREAYPEE AYIADLDAKSGASLKLTLLNPKGRI WTMVAGGGASVVYSDTICDLGGVNE LANYGEYSGAPSEQQTYDYAKTILS LMTREKHPDGKILIIGGSIANFTNV AATFKGIVRAIRDYQGPLKEHEVTI FVRRGGPNYQEGLRVMGEVGKTTGI PIHVFGTETHMTAIVGMALGHRPIP NQPPTAAHTANFLLNASGSTSTPAP SRTASFSESRADEVAPAKKAKPAMP QDSVPSPRSLQGKSTTLFSRHTKAI VWGMQTRAVQGMLDFDYVCSRDEPS VAAMVYPFTGDHKQKFYWGHKEILI PVFKNMADAMRKHPEVDVLINFASL RSAYDSTMETMNYAQIRTIAIIAEG IPEALTRKLIKKADQKGVTIIGPAT VGGIKPGCFKIGNTGGMLDNILASK LYRPGSVAYVSRSGGMSNELNNIIS RTTDGVYEGVAIGGDRYPGSTFMDH VLRYQDTPGVKMIVVLGEIGGTEEY KICRGIKEGRLTKPIVCWCIGTCAT MFSSEVQFGHAGACANQASETAVAK NQALKEAGVFVPRSFDELGEIIQSV YEDLVANGVIVPAQEVPPPTVPMDY SWARELGLIRKPASFMTSICDERGQ ELIYAGMPITEVFKEEMGIGGVLGL LWFQKRLPKYSCQFIEMCLMVTADH GPAVSGAHNTIICARAGKDLVSSLT SGLLTIGDRFGGALDAAAKMFSKAF DSGIIPMEFVNKMKKEGKLIMGIGH RVKSINNPDMRVQILKDYVRQHFPA TPLLDYALEVEKITTSKKPNLILNV DGLIGVAFVDMLRNCGSFTREEADE YIDIGALNGIFVLGRSMGFIGHYLD QKRLKQGLYRHPWDDISYVLPEHMS MTRTRPLEQKLISEEDLAANDILDY KDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
