Recombinant Human ACSS3, His-tagged

• Cat.No.: ACSS3-18H
• Product Overview: Recombinant Human Acyl-CoA Synthetase Short-Chain Family Member 3 Mitochondrial/ACSS3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala30-Ala686) of Human ACSS3 fused with a polyhistidine tag at the C-terminus.

Specification

• Description: Acyl-CoA Synthetase Short-Chain Family Member 3 Mitochondrial (ACSS3) is a member of the ATP-dependent AMP-binding enzyme family. ACSS3 localizes to the mitochondrion and catalyzes acetate and CoA into diphosphate and acetyl-CoA using the ATP. ACSS3 activates acetate for lipid synthesis use or for energy generation. Two isoforms of the human protein are produced by alternative splicing.
• Source: HEK293
• Species: Human
• Tag: His
• AA Sequence: AGAALRALVVPGPRGGLGGRGCRAL SSGSGSEYKTHFAASVTDPERFWGK AAEQISWYKPWTKTL ENKHSPSTRWFVEGMLNICYNAVDR HIENGKGDKIAIIYDSPVTNTKATF TYKEVLEQVSKLAGV LVKHGIKKGDTVVIYMPMIPQAMYT MLACARIGAIHSLIFGGFASKELSS RIDHVKPKVVVTASF GIEPGRRVEYVPLVEEALKIGQHKP DKILIYNRPNMEAVPLAPGRDLDWD EEMAKAQSHDCVPVL SEHPLYILYTSGTTGLPKGVIRPTG GYAVMLHWSMSSIYGLQPGEVWWAA SDLGWVVGHSYICYG PLLHGNTTVLYEGKPVGTPDAGAYF RVLAEHGVAALFTAPTAIRAIRQQD PGAALGKQYSLTRFK TLFVAGERCDVETLEWSKNVFRVPV LDHWWQTETGSPITASCVGLGNSKT PPPGQAGKSVPGYNV MILDDNMQKLKARCLGNIVVKLPLP PGAFSGLWKNQEAFKHLYFEKFPGY YDTMDAGYMDEEGYL YVMSRVDDVINVAGHRISAGAIEES ILSHGTVADCAVVGKEDPLKGHVPL ALCVLRKDINATEEQ VLEEIVKHVRQNIGPVAAFRNAVFV KQLPKTRSGKIPRSALSAIVNGKPY KITSTIEDPSIFGHV EEMLKQAVDHHHHHH
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Gene Information

• Gene Name: ACSS3 acyl-CoA synthetase short-chain family member 3 [ Homo sapiens ]
• Official Symbol: ACSS3
• Synonyms: ACSS3; acyl-CoA synthetase short-chain family member 3; acyl-CoA synthetase short-chain family member 3, mitochondrial; FLJ21963; AMP-binding enzyme, 33217
• Gene ID: 79611
• mRNA Refseq: NM_024560
• Protein Refseq: NP_078836
• MIM: 614356
• UniProt ID: Q9H6R3
• Chromosome Location: 12q21.31
• Pathway: Metabolic pathways, organism-specific biosystem; Propanoate metabolism, organism-specific biosystem; Propanoate metabolism, conserved biosystem; acetate conversion to acetyl-CoA, organism-specific biosystem; ethanol degradation II, organism-specific biosystem; ethanol degradation IV, organism-specific biosystem; oxidative ethanol degradation III, organism-specific biosystem
• Function: ATP binding; acetate-CoA ligase activity; ligase activity; nucleotide binding