Active Recombinant Human ACVR1B, Fc-His-tagged

• Cat.No.: ACVR1B-560H
• Product Overview: Active Recombinant Human ACVR1B(NP_004293.1)(Met 1-Glu 126) was expressed in HEK293, fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-126 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 μg/ml (100 μl/well) can bind human ALK-4 with a linear range of 0.0068-0.16 μg/ml.
• Molecular Mass: The recombinant human ALK4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 351 amino acids and has a predicted molecular mass of 39.6 kDa. In SDS-PAGE under reducing conditions, rhALK4/Fc monomer migrates as an approximately 46 kDa band due to glycosylation, with ~15% free Fc fragment.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACVR1B activin A receptor, type IB [ Homo sapiens ]
• Official Symbol: ACVR1B
• Synonyms: ACVR1B; activin A receptor, type IB; ACVRLK4; activin receptor type-1B; ActRIB; ALK4; SKR2; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2; ACTRIB
• Gene ID: 91
• mRNA Refseq: NM_004302
• Protein Refseq: NP_004293
• MIM: 601300
• UniProt ID: P36896