Recombinant Human Adenylate Kinase 1, His-tagged

• Cat.No.: AK1-2864H
• Product Overview: Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 23.7 kDa (214aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol.
• Concentration: 1mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AK1 adenylate kinase 1 [ Homo sapiens ]
• Synonyms: AK1; adenylate kinase 1; adenylate kinase isoenzyme 1; myokinase; OTTHUMP00000022220; OTTHUMP00000216413; ATP-AMP transphosphorylase 1; EC 2.7.4.3; AK 1
• Gene ID: 203
• mRNA Refseq: NM_000476
• Protein Refseq: NP_000467
• MIM: 103000
• UniProt ID: P00568
• Chromosome Location: 9q34.1
• Pathway: Metabolic pathways; Purine metabolism
• Function: ATP binding; adenylate kinase activity; nucleobase, nucleoside, nucleotide kinase activity; nucleotide binding; protein binding; transferase activity