Recombinant Human Adrenergic, Beta, Receptor Kinase 1, GST-tagged

• Cat.No.: ADRBK1-1019H
• Product Overview: Full-length recombinant human ADRBK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: ADRBK1 is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G protein-coupled receptors. ADRBK1 phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 102 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Purity: >90%
• Concentration: 0.1 ug/ul
• Specific Activity: 5.3 nmol/min/mg
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: CXCR4-mediated signaling events; Ca-dependent events; CaM pathway; Calmodulin induced events; Chemokine signaling pathway; Endocytosis; G alpha (q) signalling events; G-protein mediated events; GPCR downstream signaling; Glutamatergic synapse; Opioid Signalling; PLC beta mediated events; PLC-gamma1 signalling; Signalling by NGF; Signalling by NGF

Gene Information

• Gene Name: ADRBK1 adrenergic, beta, receptor kinase 1 [ Homo sapiens ]
• Official Symbol: ADRBK1
• Synonyms: ADRBK1; adrenergic, beta, receptor kinase 1; GRK2; BARK1; FLJ16718; BETA-ARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; OTTHUMP00000235171; G-protein coupled receptor kinase 2
• Gene ID: 156
• mRNA Refseq: NM_001619
• Protein Refseq: NP_001610
• MIM: 109635
• UniProt ID: P25098
• Chromosome Location: 11q13
• Function: ATP binding; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; alpha-2A adrenergic receptor binding; beta-adrenergic receptor kinase activity; nucleotide binding; protein binding; protein kinase activity; signal transducer activity