Recombinant Human AFP Protein, His-tagged

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Recombinant Human AFP Protein, His-tagged

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Cat.No. : AFP-52H
Product Overview : Recombinant Human AFP (19-609aa) Protein with His tag at C-terminus was expressed in insect cell and purified by using conventional chromatography techniques.
Description : This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Source : Insect cell
Species : Human
Tag : His
Form : Liquid
Molecular Mass : 67.5 kDa
Protein length : 19-609
AA Sequence : <ADP>RTLHR NEYGIASILD SYQCTAEISL ADLATIFFAQ FVQEATYKEV SKMVKDALTA IEKPTGDEQS SGCLENQLPA FLEELCHEKE ILEKYGHSDC CSQSEEGRHN CFLAHKKPTP ASIPLFQVPE PVTSCEAYEE DRETFMNKFI YEIARRHPFL YAPTILLWAA RYDKIIPSCC KAENAVECFQ TKAATVTKEL RESSLLNQHA CAVMKNFGTR TFQAITVTKL SQKFTKVNFT EIQKLVLDVA HVHEHCCRGD VLDCLQDGEK IMSYICSQQD TLSNKITECC KLTTLERGQC IIHAENDEKP EGLSPNLNRF LGDRDFNQFS SGEKNIFLAS FVHEYSRRHP QLAVSVILRV AKGYQELLEK CFQTENPLEC QDKGEEELQK YIQESQALAK RSCGLFQKLG EYYLQNAFLV AYTKKAPQLT SSELMAITRK MAATAATCCQ LSEDKLLACG EGAADIIIGH LCIRHEMTPV NPGVGQCCTS SYANRRPCFS SLVVDETYVP PAFSDDKFIF HKDLCQAQGV ALQTMKQEFL INLVKQKPQI TEEQLEAVIA DFSGLLEKCC QGQEQEVCFA EEGQKLISKT RAALGV<HHH HHH>
Endotoxin : <1.0 EU/μg of the protein by the LAL method.
Purity : >95% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/mL (determined by absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Gene Name : AFP alpha fetoprotein [ Homo sapiens (human) ]
Official Symbol : AFP
Synonyms : AFP; alpha fetoprotein; AFPD; FETA; HPAFP; alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin
Gene ID : 174
mRNA Refseq : NM_001134
Protein Refseq : NP_001125
MIM : 104150
UniProt ID : P02771
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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