Recombinant Human AFP Protein, His-tagged

• Cat.No.: AFP-52H
• Product Overview: Recombinant Human AFP (19-609aa) Protein with His tag at C-terminus was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 67.5 kDa
• Protein length: 19-609
• AA Sequence: RTLHRNEYGIASILDSYQCTAEISL ADLATIFFAQFVQEATYKEVSKMVK DALTAIEKPTGDEQSSGCLENQLPA FLEELCHEKEILEKYGHSDCCSQSE EGRHNCFLAHKKPTPASIPLFQVPE PVTSCEAYEEDRETFMNKFIYEIAR RHPFLYAPTILLWAARYDKIIPSCC KAENAVECFQTKAATVTKELRESSL LNQHACAVMKNFGTRTFQAITVTKL SQKFTKVNFTEIQKLVLDVAHVHEH CCRGDVLDCLQDGEKIMSYICSQQD TLSNKITECCKLTTLERGQCIIHAE NDEKPEGLSPNLNRFLGDRDFNQFS SGEKNIFLASFVHEYSRRHPQLAVS VILRVAKGYQELLEKCFQTENPLEC QDKGEEELQKYIQESQALAKRSCGL FQKLGEYYLQNAFLVAYTKKAPQLT SSELMAITRKMAATAATCCQLSEDK LLACGEGAADIIIGHLCIRHEMTPV NPGVGQCCTSSYANRRPCFSSLVVD ETYVPPAFSDDKFIFHKDLCQAQGV ALQTMKQEFLINLVKQKPQITEEQL EAVIADFSGLLEKCCQGQEQEVCFA EEGQKLISKTRAALGV
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: AFP alpha fetoprotein [ Homo sapiens (human) ]
• Official Symbol: AFP
• Synonyms: AFP; alpha fetoprotein; AFPD; FETA; HPAFP; alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin
• Gene ID: 174
• mRNA Refseq: NM_001134
• Protein Refseq: NP_001125
• MIM: 104150
• UniProt ID: P02771