Recombinant Human AGA protein, His-tagged

• Cat.No.: AGA-27H
• Product Overview: Recombinant human AGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: AGA, as known as N (4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1, belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily. This protein consists of different S100 proteins and then plays various roles in regulation of protein phosphorylation, the dynamics of cytoskeleton constituents, transcription factors, enzyme activities, cell growth and differentiation, and inflammatory response.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 35.7kDa (332aa)
  18-57kDa (SDS-PAGE under reducing conditions.)
• AA Sequence: ADPSSPLPLV VNTWPFKNAT EAAWRALASG GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCIHHHH HH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: AGA aspartylglucosaminidase [ Homo sapiens ]
• Official Symbol: AGA
• Synonyms: AGA; aspartylglucosaminidase; ASRG; glycosylasparaginase; aspartylglucosylamine deaspartylase; GA; AGU
• Gene ID: 175
• mRNA Refseq: NM_000027
• Protein Refseq: NP_000018
• MIM: 613228
• UniProt ID: P20933
• Chromosome Location: 4q34.3
• Pathway: Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Other glycan degradation, organism-specific biosystem; Other glycan degradation, conserved biosystem
• Function: N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; hydrolase activity; protein self-association