| Description : |
This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
174.6 kDa |
| AA Sequence : |
MGHSKQIRILLLNEMEKLEKTLFRL EQGYELQFRLGPTLQGKAVTVYTNY PFPGETFNREKFRSLDWENPTERED DSDKYCKLNLQQSGSFQYYFLQGNE KSGGGYIVVDPILRVGADNHVLPLD CVTLQTFLAKCLGPFDEWESRLRVA KESGYNMIHFTPLQTLGLSRSCYSL ANQLELNPDFSRPNRKYTWNDVGQL VEKLKKEWNVICITDVVYNHTAANS KWIQEHPECAYNLVNSPHLKPAWVL DRALWRFSCDVAEGKYKEKGIPALI ENDHHMNSIRKIIWEDIFPKLKLWE FFQVDVNKAVEQFRRLLTQENRRVT KSDPNQHLTIIQDPEYRRFGCTVDM NIALTTFIPHDKGPAAIEECCNWFH KRMEELNSEKHRLINYHQEQAVNCL LGNVFYERLAGHGPKLGPVTRKHPL VTRYFTFPFEEIDFSMEESMIHLPN KACFLMAHNGWVMGDDPLRNFAEPG SEVYLRRELICWGDSVKLRYGNKPE DCPYLWAHMKKYTEITATYFQGVRL DNCHSTPLHVAEYMLDAARNLQPNL YVVAELFTGSEDLDNVFVTRLGISS LIREAMSAYNSHEEGRLVYRYGGEP VGSFVQPCLRPLMPAIAHALFMDIT HDNECPIVHRSAYDALPSTTIVSMA CCASGSTRGYDELVPHQISVVSEER FYTKWNPEALPSNTGEVNFQSGIIA ARCAISKLHQELGAKGFIQVYVDQV DEDIVAVTRHSPSIHQSVVAVSRTA FRNPKTSFYSKEVPQMCIPGKIEEV VLEARTIERNTKPYRKDENSINGTP DITVEIREHIQLNESKIVKQAGVAT KGPNEYIQEIEFENLSPGSVIIFRV SLDPHAQVAVGILRNHLTQFSPHFK SGSLAVDNADPILKIPFASLASRLT LAELNQILYRCESEEKEDGGGCYDI PNWSALKYAGLQGLMSVLAEIRPKN DLGHPFCNNLRSGDWMIDYVSNRLI SRSGTIAEVGKWLQAMFFYLKQIPR YLIPCYFDAILIGAYTTLLDTAWKQ MSSFVQNGSTFVKHLSLGSVQLCGV GKFPSLPILSPALMDVPYRLNEITK EKEQCCVSLAAGLPHFSSGIFRCWG RDTFIALRGILLITGRYVEARNIIL AFAGTLRHGLIPNLLGEGIYARYNC RDAVWWWLQCIQDYCKMVPNGLDIL KCPVSRMYPTDDSAPLPAGTLDQPL FEVIQEAMQKHMQGIQFRERNAGPQ IDRNMKDEGFNITAGVDEETGFVYG GNRFNCGTWMDKMGESDRARNRGIP ATPRDGSAVEIVGLSKSAVRWLLEL SKKNIFPYHEVTVKRHGKAIKVSYD EWNRKIQDNFEKLFHVSEDPSDLNE KHPNLVHKRGIYKDSYGASSPWCDY QLRPNFTIAMVVAPELFTTEKAWKA LEIAEKKLLGPLGMKTLDPDDMVYC GIYDNALDNDNYNLAKGFNYHQGPE WLWPIGYFLRAKLYFSRLMGPETTA KTIVLVKNVLSRHYVHLERSPWKGL PELTNENAQYCPFSCETQAWSIATI LETLYDLTRTRPLEQKLISEEDLAA NDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
