Active Recombinant Human AHCY protein, His-tagged

• Cat.No.: AHCY-99H
• Product Overview: Active Recombinant Human AHCY protein(P23526)(2-432 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-432 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Adenosylhomocysteinase (S-adenosylhomocysteine synthase, S-adenosylhomocysteine hydrolase, adenosylhomocysteine hydrolase, S-adenosylhomocysteinase, SAHase, AdoHcyase, AHCY) is an enzyme that converts S-adenosylhomocysteine to homocysteine and adenosine. The enzyme contains one tightly bound NAD per subunit. Besides, Major Histocompatibility Complex Class I B (MHCB) has been identified as an interactor of AHCY, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AHCY and recombinant human MHCB. Briefly, AHCY were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCB-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-AHCY pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of AHCY and MHCB was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 51.3kDa
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: AHCY adenosylhomocysteinase [ Homo sapiens ]
• Official Symbol: AHCY
• Synonyms: AHCY; adenosylhomocysteinase; S adenosylhomocysteine hydrolase; SAHH; adoHcyase; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase
• Gene ID: 191
• mRNA Refseq: NM_000687
• Protein Refseq: NP_000678
• MIM: 180960
• UniProt ID: P23526