Recombinant Human AMFR protein, His-tagged

• Cat.No.: AMFR-3763H
• Product Overview: Recombinant Human AMFR protein(301-643 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 301-643 aa
• AA Sequence: LFHEVQRRIRRHKNYLRVVGNMEAR FAVATPEELAVNNDDCAICWDSMQA ARKLPCGHLFHNSCLRSWLEQDTSC PTCRMSLNIADNNRVREEHQGENLD ENLVPVAAAEGRPRLNQHNHFFHFD GSRIASWLPSFSVEVMHTTNILGIT QASNSQLNAMAHQIQEMFPQVPYHL VLQDLQLTRSVEITTDNILEGRIQV PFPTQRSDSIRPALNSPVERPSSDQ EEGETSAQTERVPLDLSPRLEETLD FGEVEVEPSEVEDFEARGSRFSKSA DERQRMLVQRKDELLQQARKRFLNK SSEDDAASESFLPSEGASSDPVTLR RRMLAAAAERRLQKQQTS
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: AMFR autocrine motility factor receptor, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: AMFR
• Synonyms: AMFR; autocrine motility factor receptor, E3 ubiquitin protein ligase; autocrine motility factor receptor; gp78; RNF45; RING finger protein 45; GP78
• Gene ID: 267
• mRNA Refseq: NM_001144
• Protein Refseq: NP_001135
• MIM: 603243
• UniProt ID: P26442