Recombinant Human ANGPTL3 protein, His-Avi-tagged

• Cat.No.: ANGPTL3-951H
• Product Overview: Recombinant Human ANGPTL3 protein(Q9Y5C1)(Ser17-Lys219), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ser17-Lys219
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ANGPTL3, His Tag at 1 μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ANGPTL3 angiopoietin-like 3 [ Homo sapiens ]
• Official Symbol: ANGPTL3
• Synonyms: ANGPTL3; angiopoietin-like 3; ANGPT5; angiopoietin-related protein 3; angiopoietin 5; ANG-5; FHBL2
• Gene ID: 27329
• mRNA Refseq: NM_014495
• Protein Refseq: NP_055310
• MIM: 604774
• UniProt ID: Q9Y5C1