Recombinant Human ANP32B protein, GST-tagged

• Cat.No.: ANP32B-9686H
• Product Overview: Recombinant Human ANP32B protein(1-177 aa), fused with N-terminal GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-177 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 8.0). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 100 mM reduced glutathione (GSH).
• AASequence: MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 12 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: ANP32B acidic (leucine-rich) nuclear phosphoprotein 32 family, member B [ Homo sapiens ]
• Official Symbol: ANP32B
• Synonyms: ANP32B; acidic (leucine-rich) nuclear phosphoprotein 32 family, member B; acidic leucine-rich nuclear phosphoprotein 32 family member B; acidic protein rich in leucines; APRIL; PHAPI2; SSP29; silver-stainable protein SSP29; putative HLA-DR-associated protein I-2
• Gene ID: 10541
• mRNA Refseq: NM_006401
• Protein Refseq: NP_006392
• UniProt ID: Q92688