Recombinant Human ANXA10 293 Cell Lysate

Cat.No. : ANXA10-8838HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for annexin A10 (ANXA10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ANXA10 annexin A10 [ Homo sapiens ]
Official Symbol : ANXA10
Synonyms : ANXA10; annexin A10; ANX14; annexin 14; annexin-10; annexin-14;
Gene ID : 11199
mRNA Refseq : NM_007193
Protein Refseq : NP_009124
MIM : 608008
UniProt ID : Q9UJ72
Chromosome Location : 4q32.3
Function : calcium ion binding; calcium-dependent phospholipid binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (3)

Ask a question
Is ANXA10 protein localized in a specific subcellular compartment? 10/20/2020

ANXA10 protein is primarily known to localize to the cytoplasmic compartment of cells. It can also associate with cell membranes in some instances. However, the subcellular localization of ANXA10 may be cell type-specific and can vary depending on different physiological conditions.

Is ANXA10 protein involved in calcium signaling? 02/09/2019

Annexin proteins, including ANXA10, have been shown to interact with calcium ions and are involved in calcium signaling pathways. They can regulate calcium fluxes across membranes and participate in calcium-mediated processes such as neurotransmitter release, muscle contraction, and cell migration. While there is limited information specifically regarding ANXA10's role in calcium signaling, it is possible that it may contribute to these processes.

Is ANXA10 protein expressed in all tissues? 11/19/2017

The expression of ANXA10 protein has been reported to vary across different tissues. It appears to be more abundant in certain tissues, such as the liver and pancreas, while its expression in other tissues may be lower or undetectable.

Customer Reviews (4)

Write a review
Reviews
07/23/2021

    The outstanding level of customer service adds extra reassurance and confidence in using the ANXA10 protein for my scientific investigations.

    05/25/2021

      The ANXA10 protein surpasses expectations in terms of quality and will undoubtedly fulfill my experimental requirements.

      07/26/2017

        With the ANXA10 protein and its accompanying technical assistance, I am confident that I can achieve successful outcomes for my research endeavors.

        05/11/2016

          the manufacturer of ANXA10 provides exceptional technical support, offering valuable assistance in troubleshooting and experimental design.

          Ask a Question for All ANXA10 Products

          Required fields are marked with *

          My Review for All ANXA10 Products

          Required fields are marked with *

          0

          Inquiry Basket

          cartIcon
          logo

          FOLLOW US

          Terms and Conditions        Privacy Policy

          Copyright © 2024 Creative BioMart. All Rights Reserved.

          Contact Us

          • /

          Stay Updated on the Latest Bioscience Trends