Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human AP1G1 293 Cell Lysate

Cat.No. : AP1G1-8819HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AP1G1 adaptor-related protein complex 1, gamma 1 subunit [ Homo sapiens ]
Official Symbol : AP1G1
Synonyms : AP1G1; adaptor-related protein complex 1, gamma 1 subunit; ADTG, CLAPG1; AP-1 complex subunit gamma-1; gamma1 adaptin; gamma adaptin; gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; adaptor protein complex AP-1 subunit gamma-1; golgi adaptor HA1/AP1 adaptin subunit gamma-1; adapter-related protein complex 1 subunit gamma-1; clathrin assembly protein complex 1 gamma large chain; clathrin assembly protein complex 1 gamma-1 large chain; clathrin-associated/assembly/adaptor protein, large, gamma 1; ADTG; CLAPG1; MGC18255;
Gene ID : 164
mRNA Refseq : NM_001030007
Protein Refseq : NP_001025178
MIM : 603533
UniProt ID : O43747
Chromosome Location : 16q23
Pathway : Clathrin derived vesicle budding, organism-specific biosystem; Disease, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; HIV Infection, organism-specific biosystem; Host Interactions of HIV factors, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem;
Function : kinesin binding; protein binding; protein transporter activity; transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (17)

Ask a question
What are the important applications of AP1G1 in scientific research? 12/22/2022

AP1G1 can be used to study the mechanism of tumor occurrence and development, immune regulation, protein structure and function, and has important scientific application value.

What is the action mechanism of AP1G1 ? 10/17/2022

Recombinant AP1G1 regulates signal transduction and metabolic pathways of target cells primarily by binding to specific receptors, and is involved in a range of biological processes.

Which application prospect of AP1G1 in cancer therapy? 06/11/2022

AP1G1 has a certain application prospect in cancer therapy, it can participate in the regulation of tumor cell proliferation, apoptosis and other processes, and may be used in the development of new drugs.

Does the stability of AP1G1 ? 03/01/2022

The AP1G1 is highly stable and can maintain its biological activity and effect under different conditions.

What is the role of AP1G1 in the treatment of diabetes? 11/22/2021

Recombinant AP1G1 is involved in the regulation of insulin secretion and glucose metabolism, so it has certain application prospects in the treatment of diabetes.

What is the application status of recombinant AP1G1 in clinical trials? 10/13/2021

The recombinant AP1G1 protein has made some progress in clinical trials, but its application still needs more research and evaluation.

How the clinical application of AP1G1 ? 06/10/2021

AP1G1 can be used to treat diseases such as tumors, diabetes, autoimmune diseases, and can also be used for research in the field of biotechnology.

What structural characteristics of AP1G1 ? 10/13/2020

The structure of the AP1G1 is characterized by a complex of multiple protein subunits that form a stable three-dimensional structure through specific interactions.

What are the pharmacokinetic characteristics of AP1G1 ? 08/04/2020

The absorption, metabolism and excretion characteristics of AP1G1 in vivo still need to be further studied and analyzed.

What is the synthesis method of AP1G1 ? 05/14/2020

The synthesis of AP1G1 can be completed by cloning, expression, purification and structural analysis of foreign genes.

What is the application prospect of recombinant AP1G1 in the field of biotechnology? 03/24/2020

AP1G1 can be used as an important biomolecular tool to study protein structure and function, and has broad application prospects.

What is the impact of the difference between recombinant AP1G1 and natural AP1G1? 03/22/2020

Differences between the recombinant AP1G1 protein and the natural AP1G1 protein may affect its biological activity and effect, and further experimental and clinical testing is needed to evaluate it.

How effective is recombinant AP1G1 compared to other treatments? 11/29/2019

The effects of AP1G1 compared to other therapeutic approaches need to be further evaluated in more experimental and clinical studies.

What is the relationship between recombinant AP1G1 and autoimmune diseases? 09/24/2019

Recombinant AP1G1 may play a role in the treatment of autoimmune diseases by regulating the activity and function of immune cells.

What are the purification methods of AP1G1 ? 07/04/2019

The purification methods of AP1G1 include affinity chromatography, ion exchange chromatography, gel filtration chromatography and other technologies.

Are there other s with similar mechanisms of action to AP1G1 s? 05/30/2019

At present, many s are known to have similar mechanisms of action, but their specific differences need further research and analysis.

Is it feasible to combine recombinant AP1G1 with other drugs in tumor therapy? 03/24/2019

The recombinant AP1G1 protein can be used in combination with other drugs, but further experimental and clinical studies are needed to determine whether this is feasible.

Customer Reviews (4)

Write a review
Reviews
03/06/2022

    The short half-life and high clearance of AP1G1 enable it to penetrate the tissue better, reach the lesion site, and improve the therapeutic effect.

    01/21/2022

      Production process of this recombinant protein is very environmentally friendly, from the selection of raw materials to the production process reflects the concept of green production.

      10/12/2020

        I appreciate the quality of this recombinant protein manufacturer's attention to the product, and their product stability is very good, which makes our experiment more smooth.

        09/18/2020

          The strip shape of AP1G1 was regular in Western blot, showing good quality.

          Ask a Question for All AP1G1 Products

          Required fields are marked with *

          My Review for All AP1G1 Products

          Required fields are marked with *

          0

          Inquiry Basket

          cartIcon
          logo

          FOLLOW US

          Terms and Conditions        Privacy Policy

          Copyright © 2024 Creative BioMart. All Rights Reserved.

          Contact Us

          • /

          Stay Updated on the Latest Bioscience Trends